There remains a lot that we still poorly understand about the inner workings of the genetic code. That introns are persuasive in the genetics of higher life forms and almost uniquely associated with high temperature bacteria is obviously perplexing.
Perhaps it is high time to rethink the simple models we have relied on for the past few decades. Or at least stop simply dismissing aspects of the genome as irrelevant.
We may simply have to keep gathering data for a generation or so. There is nothing wrong with that at all and is the honorable profession of science.
Bacteria From Hot Springs
by Staff Writers
A bacteria that lives in
Biochemists Alan Lambowitz and Georg Mohr began investigating Thermosynechococcus elongatus, a cyanobacterium that can survive at temperatures up to 150 degrees Fahrenheit, after they noticed an unusually high percentage of the bacteria's genetic sequence was composed of elements known as group II introns.
"Introns are mysterious elements in evolution," says Lambowitz, a professor of molecular biology and director of the Institute of Molecular
"Until the 1970s it was believed that genes in all organisms would be continuous and that they would make a continuous RNA, which would then get translated into a continuous protein. It was found, however, that most genes of the eukaryotes, the higher organisms including humans, aren't like that at all. Most of the genes in higher organism are discontinuous. They consist of DNA coding regions that are separated by areas known as introns.
"Genomes become loaded down with these introns, which are thought to have evolved from genomic parasites that existed for their own benefit and could spread without killing the host organism," says Lambowitz.
"It remains a major question in evolution as to why these introns exist, and how they came to compose such a large part of the human genome."
In order to better understand the early history of introns, Lambowitz and Mohr have focused their investigation on bacteria because they're believed to be the original evolutionary wellspring of the introns. They're looking at T. elongatus in particular because it's the only known bacteria in which introns have proliferated in a manner similar to that in higher organisms, such as humans.
"We can't go back a billion years in a time machine to see how introns proliferated in the early eukaryotes," says Mohr, a research scientist in Lambowitz's lab. "What we can do is investigate the mechanisms that have allowed introns to proliferate in this organism, and try to infer how they evolved in eukaryotes, like humans, in which as much as 40 percent of the genome is made up of introns."
Among the mechanisms they've identified, perhaps the most surprising has been that heat plays a significant role in allowing introns to proliferate in T. elongatus. High temperatures, like those found in the hot springs
This evidence of "DNA melting," says Lambowitz, is particularly suggestive when trying to imagine how introns proliferated in early eukaryotes, because the earth was hotter a billion or so years ago, when the early eukaryotes emerged. The genomes of the early eukaryotes may have begun with only a few introns, but over time, thanks in part to the high temperatures, the introns could have proliferated rapidly.
Lambowitz and Mohr's investigation of introns in T. elongatus may also, unexpectedly, prove an enormous boon to researchers who are trying to use other high-temperature ("thermophilic") bacteria to improve the efficiency of biofuels.
"There's one bacterial species in particular," says Lambowitz, "which lives at high temperature and is very good at converting cellulose toethanol, but has been intractable to genetic manipulation. The Department of Energy has a considerable amount of money invested in it, and they need to improve the strains but haven't been able to do it. When we discovered these thermophilic introns, which work better at high temperatures, we were able to adapt them pretty rapidly for gene targeting."
The technology for using group II introns in gene targeting, known as targetron technology, was pioneered by Lambowitz and his coworkers. Lambowitz and Mohr are already working with scientists at Oak Ridge
They also foresee applying what they've discovered about T. elongatus introns and temperature to a whole range of biotech and biomedical applications that involve organisms and enzymes that function best at high temperatures. However, they are still planning to delve further into the more profound, basic scientific questions that drew them to the subject in the first place.
