Showing posts with label lignin. Show all posts
Showing posts with label lignin. Show all posts

Wednesday, May 13, 2009

Cellulosic Biofuel update

If I am reading this right, we can now accept a full range of cellulose containing feedstocks that pass first through a pretreatment phase. Nothing is said about this except to say that it is mild and surely wet. This treated feedstock is then passed into the bio-processing phase to brew up its cellulose into ethanol. It appears that the lignin fraction separates and can be recovered for separate processing.

This sounds like it can be set up easily in the farm environment allowing for the production of a shippable brew or more likely the ethanol itself.

The productivity is the core of this announcement. It was unexpected so soon although efforts are in that direction.

The lignin is also prospective for conversion but can easily be shipped. We will want to separate ethanol and process the balance of the brew itself. The process should lend itself to having the remaining solids passed back into the brewing process with a new charge.

Right now it sounds like a slow but effective process, not unlike wine making.

May 08, 2009
"This is a true breakthrough that takes us much, much closer to billions of gallons of low cost cellulosic biofuels," said Michigan State University's Dr. Bruce Dale, who is also Editor of the journal Biofuels, Bioproducts and Biorefineries.
"Many had thought that CBP was years or even decades away, but the future just arrived. Mascoma has permanently changed the biofuels landscape from here on."
Consolidated bioprocessing, or CBP, harnesses the power of nature’s best celluose utilizing and ethanol fermenting microbes and allows nature to do the majority of the work resulting in a simpler process consisting of a mild pretreatment followed by the introduction of microbes that both hydrolyze and ferment the sugars into ethanol;
Thermophilic Bacteria -- Production of nearly 6% wt/vol ethanol by an engineered thermophilie, an increase of 60% over what was reported just a year ago;
-- The first report of targeted metabolic engineering of a cellulose-fermenting thermophile, Clostridium thermocellum, leading to a reduced production of unwanted organic acid byproducts;
and -- Selected strains of C. thermocellum that can rapidly consume cellulose with high conversion and no added cellulase, and grow on cellulose in the presence of commercial levels of ethanol.
Recombinant, Cellulolytic Yeast
-- 3,000-fold increase in cellulase expression;
-- A significant 2.5-fold reduction in the added cellulase required for conversion of pretreated hardwood to ethanol; and -- Complete elimination of added cellulase for conversion of waste paper sludge to ethanol. In February 2009, Mascoma announced that its pilot facility in Rome, NY had begun producing cellulosic ethanol. The demonstration facility, which was constructed with the generous support from the State of New York through the NYS Department of Agriculture & Markets and the New York State Energy Research and Development Authority, has the flexibility to run on numerous biomass feedstocks including wood chips, tall grasses, corn stover (residual corn stalks) and sugar cane bagasse.
The facility will provide process performance engineering data sufficient to support construction of 1/10th scale and commercial scale biorefineries in Kinross, MI, with support from the Department of Energy and State of Michigan.

Thursday, March 5, 2009

Xylose Enzyme Discovered

Step by step we are finding ways to convert cellulose into a usable biofuel like ethanol. This company is also attempting to produce a better biofuel that ethanol itself. Their present focus is on biobutanol.

To date most effort, for good reason has gone into simply unraveling cellulose into constituent sugars and lignin. As these are freeing up, it then becomes necessary to process the derivative products. We have already reported on work on lignin and this is one of the resulting sugars from the processing of cellulose..

It does look as if we will establish protocols and pathways to convert cellulose into a desirable biofuel. The magic question then becomes whether we can do it profitably.

The multiple processes and separation steps are somewhat discouraging, particularly when you will also have to fine tune the feedstock. However, uniform feedstocks such as corn stover and cattail waste and bagasse are all excellent sources of renewable feedstocks.


Researcher discovers enzyme to ferment xylose

By Anna Austin

Web exclusive posted Feb. 17, 2009, at 3:45 p.m. CST

http://www.ethanolproducer.com/article.jsp?article_id=5390

Eckhard Boels, cofounder of Swiss biofuel company Butalco gmbH and a professor at Goethe-University in Frankfurt, Germany, has discovered a new enzyme which teaches yeast cells to ferment xylose into ethanol. Xylose is an unused waste sugar in the cellulosic ethanol production process.

According to Boles, one of the major problems with cellulosic ethanol is that when utilizing other parts of plants, which today are considered waste, yeasts are unable to ferment some of the sugars in a majority of the plant material.

Saccharomyces cerevisiae (SC), a yeast commonly used for ethanol production, lacks the ability to ferment some sugars. “Heterologous expression of a xylose isomerase would enable yeast cells to metabolize xylose,” Boles said. “However, many attempts to express a prokaryotic xylose isomerase with high activity in SC have failed so far. We have screened nucleic acid databases for sequences encoding putative xylose isomerases, and finally could clone and successfully express a highly active new kind of xylose isomerase from an anaerobic bacterium in SC.”

The new enzyme was taken from the bacterial organism and inserted into yeast cells that were retrieved from a commercial ethanol plant. “With just a minor effort, we were able to teach the yeast cells how to ferment the xylose into ethanol,” Boles said.

Boles believe the findings may provide an excellent starting point for further improvement of xylose fermentation in industrial yeast strains, and greatly enhance the development of an efficient biomass-to-ethanol fermentation process. His company, Butalco gmbH, is now working to construct yeast strains to convert plant waste materials into biobutanol.

The research was published in the
Applied and Environmental Microbiology journal in February.